siRNA / miRNA gene silencing Human HT-1376 (urinary bladder cell line)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Rat - Point mutation H9C2 CELF1

Get tips on using Collagen I antibody to perform Western blotting Type I collagen

Get tips on using Anti-Collagen III antibody (ab7778) to perform Western blotting Type III collagen

Get tips on using Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat Anti-Type III Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Anti-Collagen I antibody [COL-1] (ab90395) to perform Western blotting Type I collagen

Get tips on using Goat Anti-Type III Collagen-BIOT to perform Immunohistochemistry Collagen Type III - Goat Mouse Biotin

Get tips on using Monoclonal Anti-Collagen, Type III antibody produced in mouse to perform Western blotting Type III collagen